BIOL 2104 - Biotechnology I

Description
The second course in a three-part series in the biotechnology program. The course stresses the theory and practice of separation techniques and safety procedures that would be employed in the purification and assay of such biomolecules as nucleic acids, proteins, and other related substances and the relationship of these molecules to living organisms. An emphasis is placed on the use of laboratory tools and equipment to familiarize the student with current biochemical techniques. Three hours of lecture and three hours of lab weekly.

Pre-Requisite
NONE

4 Credit Hour(s)

Contact Hours
45 lecture contact hours. 45 laboratory hours

5 Faculty Load Hour(s)

Semesters Offered
Spring

ACTS Equivalent
N/A

Grade Mode
A-F

Learning Outcomes
Students completing this course will:

Demonstrate proper scientific laboratory record keeping.
Identify and calculate concentration of selected products.
Determine type of separation method to isolate product.
Explain the process of isolating products from microorganisms and the applications.
Demonstrate the process of proper handling genetically engineered organisms and utilize all necessary safeguards.
Apply the principles learned to comprehend, evaluate, and solve problems observed in biological chemical isolation and purification.

General Education Outcomes Supported
N/A

Standard Practices
Topics list

Lab safety
Biochemistry
Basic calculations for preparing solutions
Bioreactors
Column separation
Chromatography
- Identification
- Separation
Spectrophotometry
Membrane separation
Membrane sterilization
Applications of identification and purification

Learning activities

Courses must, at a minimum, cover the core learning outcomes for each topic.
Laboratory exercises should include chromatography, utilization of bioreactors, spectrophotometry, membrane separation, column separation, and membrane sterilization.

Assessments

Minimum requirements:

Written exams that include higher order thinking questions
Lab notebooks/write-ups
Final exam that includes some comprehensive questions.

Grading guidelines

A minimum of 70% of the grade must be proctored, supervised, or otherwise verified.
Approximately 25% of the grade must come from lab work since the lab and lecture credits for this course are combined.