BIOL 2204 - Biotechnology II

Description
The third course in a three-part series, stresses the theory and practice of current techniques used in DNA science, protein isolation, and immunology and introduces selected biotechnology protocols. An emphasis is placed on the use of laboratory tools and equipment to familiarize the student with current molecular biology techniques. Three hours of lecture and three hours of lab weekly.

Pre-Requisite
NONE

4 Credit Hour(s)

Contact Hours
45 lecture contact hours. 45 laboratory hours

5 Faculty Load Hour(s)

Semesters Offered
Fall

ACTS Equivalent
N/A

Grade Mode
A-F

Learning Outcomes
Students completing this course will:

Demonstrate proper scientific laboratory record keeping.
Describe the process of genetically modifying an organism and the applications.
Demonstrate proper handling of genetically engineered organisms and utilize all necessary safeguards.
Create a genetically modified organism designed to produce a product.
Identify which sample contains modified DNA or a specific protein.
Apply the principles learned to comprehend, evaluate and solve problems observed in isolation and purification of specific proteins.

General Education Outcomes Supported
N/A

Standard Practices
Topics list

Lab safety
Basic calculations for preparing solutions
Genetic modification techniques
Isolation of DNA
PCR
Agarose gel electrophoresis
Plasmid engineering
Restriction enzyme digestion and purification
Cell transformation and screening for clones
Protein induction and expression
Polyacrylamide gel electrophoresis
Protein purification
Antigen/antibody assays
Cell culture
Applications of identification and purification

Learning activities

Courses must, at a minimum, cover the core learning outcomes for each topic.
Laboratory exercises should include DNA digestions, DNA ligations, Agarose electrophoresis, PCR, clonal selection, Protein purification, polyacrylamide electrophoresis, antibody/antigen assays, and cell culturing.

Assessments

Minimum requirements:

Written exams that include higher order thinking questions
Lab notebooks/write-ups
Final exam that includes some comprehensive questions.

Grading guidelines

A minimum of 70% of the grade must be proctored, supervised, or otherwise verified.
Approximately 25% of the grade must come from lab work since the lab and lecture credits for this course are combined.